BACTERIUM STRAIN SPHINGOBACTERIUM MIZUTAE-32 AS PRODUCER OF SPML RESTRICTION ENDONUCLEASE, RECOGNIZING AND CLEAVING 5'-AT'CGAT-3' NUCLEOTIDE SEQUENCE Russian patent published in 2005 - IPC

Abstract RU 2266323 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to Bacterium strain sphingobacterium Mizutae-32 producing restriction endonuclease named as Spml, which recognizes and cleaves 5'-AT'CGAT-3' nucleotide sequence. Strain is isolated from melt water in process of searching endonuclease producing strain with high activity at low temperatures. Strain is capable of growth at 6°C, is stable for two week storage at 20°C. Strain-produced enzyme hydrolyzes phage-lambda DNA and T7 at 6-10°C. Enzyme yield is 300000 U/10 g of cells.

EFFECT: effective strain for restriction endonuclease production.

2 dwg, 1 tbl, 4 ex

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RU 2 266 323 C2

Authors

Puchkova L.I.

Radionenko Ju.V.

Ushakova T.A.

Repin V.E.

Dates

2005-12-20—Published

2003-06-16—Filed