STRAIN OF BACTERIUM BACILLUS COAGULANS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5′-GATNNNN-ATC-3′ Russian patent published in 1996 - IPC

Abstract RU 2057806 C1

FIELD: microbiology, biotechnology, genetic engineering. SUBSTANCE: new strain of Bacillus coagulans (ВКПМ B-6670) is proposed as a producer of restriction endonuclease Bco631 I. Strain is isolated from thermal sources as a result of screening. Enzyme yield is 6000 U/g wet biomass, specific activity of the end enzyme is 10000 U/ml. Restrictase Bco631 I is an isoschizomer of restrictase Bsa BI and can replace the latter in all genetic engineering investigations. EFFECT: new strain of bacterium indicated above, high yield of the end enzyme. 1 dwg

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RU 2 057 806 C1

Authors

Repin V.E.

Tereshchenko T.A.

Lebedev L.R.

Dates

1996-04-10—Published

1994-05-17—Filed