STRAIN OF BACTERIUM BACILLUS SCHLEGELII - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5'-CCNNNNNNNGG-3' Russian patent published in 1997 - IPC

Abstract RU 2073717 C1

FIELD: genetic engineering, microbiology, biotechnology. SUBSTANCE: invention relates to new strain of Bacillus schlegelii ("ВКПМ" B-6310) - a producer of restrictase Bsc4 I isolated from soil as result of restrictase producers search. Enzyme yield is 5000 U/g wet biomass, specific activity of enzyme is 10000 U/ml. Restrictase Bsc4 I is an isoschizomer of restrictase BsiY I and can replace the latter in all genetic engineering works. Restrictase Bsc4 I shows thermostability and able to recognize and split the nucleotide sequence 5'-CCNNNNNNNGG-3'. EFFECT: new strain indicated above, high yield and specific activity of enzyme. 1 dwg

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RU 2 073 717 C1

Authors

Repin V.E.

Tereshchenko T.A.

Lebedev L.R.

Dates

1997-02-20—Published

1994-02-08—Filed