STRAIN OF BACTERIUM BACILLUS SPECIES - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5'-(A/G)-CCGG-(T/C)-3' Russian patent published in 1995 - IPC

Abstract RU 2037522 C1

FIELD: biotechnology. SUBSTANCE: detection of strain-producer Bacillus species providing preparing thermostable restriction endonuclease which can recognize and cleave nucleotide sequence 5′-(A/G)-CCGG-(T/C)-3′ with high yield and activity of enzyme. Strain Bacillus species ВКПМ-B5941 is isolated from thermal sources after screening of site-specific endonuclease restriction (restrictases). The yield of enzyme is 4000 U/g wet biomass, specific activity of the end enzyme is 10000 U/ml. Restrictase Bse 118I is an isoschizomer of restrictase Cfr 10I and can substitute the latter in all genetic-engineering studies. EFFECT: increased yield of enzyme.

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RU 2 037 522 C1

Authors

Repin Vladimir Evgen'Evich

Dates

1995-06-19—Published

1992-07-06—Filed