FIELD: biotechnology, genetic engineering, medicine, immunology.
SUBSTANCE: invention proposes recombinant plasmid DNA pBMC-RT(A)m-1. Plasmid comprises the reverse human immunodeficiency virus transcriptase gene of type 1, subtype A, wherein in codons of their nucleotide sequence with predominance of adenine and/or thymine adenine and/or thymine are replaced for guanine and/or cytosine partially. Plasmid provides high level of expression of the human immunodeficiency reverse transcriptase in eucaryotic cells. The proposed plasmid can be used for immunization against human immunodeficiency virus of type 1 (HIV-1), subtype 2.
EFFECT: valuable biological and medicinal properties of plasmid.
1 tbl, 3 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMID DNA pBMC-RT(A)-hum FOR EXPRESSING PROTEIN OF REVERSE TRANSCRIPTASE OF HUMAN IMMUNODEFICIENCY VIRUS | 2006 |
|
RU2355764C2 |
| RECOMBINANT PLASMID DNA PBMC-QP120(A)M-1 FOR IMMUNIZATION AGAINST HUMAN IMMUNODEFICIENCY VIRUS | 2003 |
|
RU2227161C1 |
| RECOMBINANT PLASMID DNA PBMC-GAG(A)MOD FOR EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS PROTEIN GAG IN EUCARYOTIC CELLS | 2003 |
|
RU2241752C1 |
| RECOMBINANT PLASMID DNA pBMC-nef(A)m-1 EXPRESSING PROTEIN NEF OF HUMAN IMMUNODEFICIENCY VIRUS TYPE I VIRUS IN EUCARYOTIC CELLS | 2003 |
|
RU2229518C1 |
| EXPRESSION PLASMID DNA pBMC - nef (A)-hum | 2006 |
|
RU2335540C1 |
| pBMC-gp140(A)-hum EXPRESSION PLASMA DNA | 2006 |
|
RU2346044C2 |
| EXPRESSIVE PLASMID DNA p-BMC-gag (A)-HUM FOR EXPRESSION OF PROTEIN p55 HJV-1 IN EUKARYOTE CELLS | 2006 |
|
RU2345138C2 |
| POLYPEPTIDE E117 DESIGNED FOR ANTIBODY ASSAY TO THE HUMAN IMMUNODEFICIENCY VIRUS OF THE FIRST TYPE, DNA FRAGMENT HE117 ENCODING POLYPEPTIDE E117, RECOMBINANT PLASMID DNA PHE117 ENCODING POLYPEPTIDE E117, STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE E117 | 1992 |
|
RU2043413C1 |
| POLYPEPTIDE G103 DESIGNED FOR ANTIBODY ASSAY TO HUMAN IMMUNODEFICIENCY VIRUS OF THE FIRST TYPE, DNA FRAGMENT HG103 ENCODING POLYPEPTIDE G103, RECOMBINANT PLASMID DNA PHG103 ENCODING POLYPEPTIDE G103, STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE G103 | 1992 |
|
RU2043414C1 |
| POLYPEPTIDE P102 DESIGNATED FOR ANTIBODY ASSAY TO HUMAN IMMUNODEFICIENCY VIRUS TYPE-1, DNA HP102-FRAGMENT ENCODING POLYPEPTIDE P102, RECOMBINANT PLASMID DNA PH A102 ENCODING POLYPEPTIDE P102, STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE P102 | 1992 |
|
RU2071502C1 |
