FIELD: biology.
SUBSTANCE: present invention relates to biotechnology, particularly to genetic engineering and can be used in the biomedical industry. Proposed is a recombinant plasmid pBMC-RT(A)-hum, meant for expressing reverse transcriptase of the human immunodeficiency virus, and is distinguished by that, it contains an artificial gene (RT(A)-hum) synthesised by an enzyme, which is characterised by a sequence of nucleotides, optimally adapted to expression in cells of mammals.
EFFECT: use of the recombinant plasmid provides for significant increase (6-8 times) in output of the target protein compared with the primary standard.
4 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| pBMC-gp140(A)-hum EXPRESSION PLASMA DNA | 2006 |
|
RU2346044C2 |
| EXPRESSIVE PLASMID DNA p-BMC-gag (A)-HUM FOR EXPRESSION OF PROTEIN p55 HJV-1 IN EUKARYOTE CELLS | 2006 |
|
RU2345138C2 |
| EXPRESSION PLASMID DNA pBMC - nef (A)-hum | 2006 |
|
RU2335540C1 |
| RECOMBINANT PLASMID DNA PBMC-RT(A)M-1 USED FOR EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS REVERSE TRANSCRIPTASE (RT)IN HIGHER EUCARYOTIC CELLS | 2003 |
|
RU2238977C1 |
| RECOMBINANT PLASMID DNA pBMC-nef(A)m-1 EXPRESSING PROTEIN NEF OF HUMAN IMMUNODEFICIENCY VIRUS TYPE I VIRUS IN EUCARYOTIC CELLS | 2003 |
|
RU2229518C1 |
| RECOMBINANT PLASMID DNA PBMC-GAG(A)MOD FOR EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS PROTEIN GAG IN EUCARYOTIC CELLS | 2003 |
|
RU2241752C1 |
| RECOMBINANT PLASMID DNA PBMC-QP120(A)M-1 FOR IMMUNIZATION AGAINST HUMAN IMMUNODEFICIENCY VIRUS | 2003 |
|
RU2227161C1 |
| GENETIC MAKE-UPS FOR ANTI-HIV THERAPY | 2010 |
|
RU2426788C1 |
| CODON-OPTIMISED HAIPL1-ENCODING NUCLEOTIDE SEQUENCE AND EXPRESSION VECTOR CONTAINING SAID SEQUENCE | 2021 |
|
RU2785621C1 |
| NUCLEIC ACID SEQUENCE ENCODING HIV-1 GAG PROTEIN, METHOD FOR ITS PREPARING, VECTOR CONTAINING THEREOF, PROTEIN ENCODED BY ITS, PHARMACEUTICAL COMPOSITION AND THEIR USING IN PROPHYLAXIS AND/OR TREATMENT OF HIV-INFECTION AND AIDS | 2002 |
|
RU2312896C2 |
