METHOD FOR GROWING BOLETUS EDULIS MYCELIUM Russian patent published in 2004 - IPC

FIELD: agriculture, mushroom growing.

SUBSTANCE: invention relates to methods for growing Polish mushroom mycelium, Boletus edulis, and can be used in artificial growing Polish mushroom, Boletus edulis. Method for growing Boletus edulis mycelium involves seeding Polish mushroom basidiospores, Boletus edulis, in nutrient medium, their incubation up to formation the primary mycelium. The primary formed mycelium is removed and added its in capacity with nutrient medium and incubated under dark condition at temperature 20-24^oC for 30 days up to formation mycelium on medium surface s mucilaginous-fibrous mass with dense colorless film down from that spawn grows representing the thick net of branched hyphas under condition of liquid nutrient medium or as the dense substrate and felted air mycelium in agarized nutrient medium. As the nutrient medium method involves using mineral salts, sugars, amino acids, organic acids, fungal, vegetable or animal biologically active substances in different but less 1% concentrations in distilled water, pH value of media is 4.5-5.0. Medium comprises organic complex of fungal origin as a source of biologically active substances, namely 0.25 wt.-% of yeast autolysate. Nutrient medium comprises organic complex of vegetable origin - 3 wt.-% of wort as a source of biologically active substances. Nutrient medium comprises organic complex of animal origin - 1.5 wt.-% of peptone as a source of biologically active substances. Application of this method allows growing Polish mushroom mycelium, Boletus edulis, under artificial conditions, simplifying technological process in growing Polish mushroom mycelium, Boletus edulis, converting to the spawn. Also, invention provides reducing process period, reducing material consumptions for purchase of expensive reagents, devices and equipment.

EFFECT: improved growing method.

5 cl, 6 dwg, 3 ex