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Application

2003101415/13, 2003-01-21

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Start date

2003-01-21

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Actual filing date

2003-01-21

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Published

2005-01-20

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Inventor

Zhesterev V.I.Gorshkova T.F.Lapteva O.G.Balysheva V.I.Khripunov E.M.Kovalev Nikolaj AndreevichUsenja Mikhail Mikhajlovich

(73)

Holder

Vnii Veterinarnoj Virusologii I Mikrobiologii

METHOD FOR MANUFACTURING RABIES INACTIVATED VACCINE FOR FARM ANIMALS Russian patent published in 2005 - IPC

Abstract RU 2244557 C2

FIELD: biotechnology.

SUBSTANCE: the present innovation deals with biotechnology of viral preparations. One should apply RB-71 strain of rabies virus which should be introduced into culture reservoir (0.1-0.01 MLD₅₀/cell) simultaneously with VNK-21 at initial cell concentration being 0.5-0.5 mln cells/ml to be grown in suspension. Cultivation should be performed in suspension medium at 37 C for 5-6 d at constant mixing and maintaining pH value of 7.2-7.4. The obtained viral raw material at infectious 6.5-6.8 lg MLD₅₀/ml and antigenic activity being 1:90-270 should be inactivated with 1,8,36-diendomethylene-1,3,6,8-tetriasecyclodecane 0.01% at 37 C for 3 d. Ready-to-use vaccine preparation meets the requirements of the standard for veterinary preparations being of 1.5-2.0 IU activity.

EFFECT: higher efficiency.

1 ex, 1 tbl

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RU 2 244 557 C2

Authors

Zhesterev V.I.

Gorshkova T.F.

Lapteva O.G.

Balysheva V.I.

Khripunov E.M.

Kovalev Nikolaj Andreevich

Usenja Mikhail Mikhajlovich

Dates

2005-01-20—Published

2003-01-21—Filed