FIELD: genetic engineering.
SUBSTANCE: recombinant plasmid DNA p30NE2 contains DNA fragment of pasmid pQE30, including promoter/operator of T5 phage; ribosome-binding site; phage lambda transcription terminator, and β-lactomase gene as genetic marker, as well as BamHI/HindIII fragment, encoding 181-amino acid N-terminal fragment of glycoprotein E2 of classical bog cholera virus. Plasmid provides expression of glycoprotein E2 of classical bog cholera virus in E/coli bacteria cells. Invention is useful in diagnostic investigation and in vaccine production.
EFFECT: genetically engineered plasmid for vaccine preparations.
3 dwg, 6 ex
Authors
Dates
2005-04-20—Published
2003-08-05—Filed