FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology and concerns preparing a genetic construct providing a synthesis of p35d recombinant protein in Escherichia coli cells. There are presented: recombinant plasmid DNA pQE-p35d providing the synthesis of p35d recombinant protein of cowpox virus and containing in accordance with physical and genetic map presented on Fig. 2: pQE30 plasmid vector, a fragment coding MRGSHHHHHHG oligopeptice and a fragment of 17 base pairs, coding a fragment of p35 protein of cowpox virus within 1 to 239 amino acid residues (Fig.1a); Escherichia coli XL1Blue/pQE-p35d B-1252 bacterial strain that is a producer of p35d recombinant protein of cowpox virus, containing recombinant plasmid DNA pQE-p35d deposited in the Collection of Bacteria, Bacteriophages and Fungi of FBUN GNTs VB Vector, registration No. B-1252, and p35d recombinant protein of cowpox virus.
EFFECT: solutions may be used to engineer the test systems and to prepare orthopoxvirus split vaccines.
3 cl, 7 dwg, 5 ex
| Title |
Year |
Author |
Number |
| AGENT FOR NEUTRALISING SMALLPOX VIRUS |
2013 |
- Khlusevich Jana Aleksandrovna
- Tikunova Nina Viktorovna
- Morozova Vera Vital'Evna
- Bulychev Leonid Egorovich
- Bormotov Nikolaj Ivanovich
- Vlasov Valentin Viktorovich
- Sergeev Aleksandr Nikolaevich
|
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2005 |
- Dubrovskaja Viktorija Vladislavovna
- Tikunova Nina Viktorovna
- Morozova Vera Vital'Evna
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- Laman Aleksandr Georgievich
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- Brovko Fedor Aleksandrovich
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|
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2023 |
- Merkuleva Yuliya Aleksandrovna
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|
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2011 |
- Tregubchak Tat'Jana Vladimirovna
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- Shchelkunov Sergej Nikolaevich
|
RU2471869C1 |
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2009 |
- Gileva Irina Pavlovna
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- Rjazankin Igor' Aleksandrovich
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- Shchelkunova Galina Aleksandrovna
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|
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2021 |
- Merkuleva Yuliya Aleksandrovna
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|
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2015 |
- Kolosova Irina Valerevna
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- Maksyutov Rinat Amirovich
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|
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2016 |
- Yakubitskij Stanislav Nikolaevich
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|
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2021 |
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1993 |
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