FIELD: biotechnology, microbiology. SUBSTANCE: invention relates to hybridoma technology. Hybridoma R1/S-5A6 is obtained as result of fusion of murine myeloma of the strain Sp-2/0 and murine splenocytes of the strain BALB/c immunized with inactivated, purified, concentrated antigen of Rickettsia prowazekii. Monoclonal antibodies produced by hybridoma are used as component of immunoenzyme-test-systems as antibody- containing substrate in indication and identification of Rickettsia prowazekii. The resolution capacity of test-system based on monoclonal antibodies is estimated to be 6.0 x 102 of hemolytically active rickettsia in cm2 (HAR•cm-3). The use of hybridoma producing monoclonal antibodies to Rickettsia prowazekii allows to detect and identify typhus pathogen. EFFECT: valuable properties of test- system. 5 tbl, 1 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF MAMMALIAN HYBRID CELL C3/S-3E5 OF MUS MUSCULUS L. PRODUCING MONOCLONAL ANTIBODY TO BERNET'S COXIELLAS | 2003 |
|
RU2257414C1 |
| STRAIN OF HYBRID CELLS M/N-10G9 OF ANIMAL MUS MUSCULUS L PRODUCING MONOCLONAL ANTIBODIES RAISED TO MARBURG VIRUS | 2001 |
|
RU2186107C1 |
| STRAIN OF HYBRID CELLS E4/N-6 G5 OF ANIMAL MUS MUSCULUS L PRODUCING MONOCLONAL ANTIBODIES RAISED TO EBOL'S VIRUS | 2001 |
|
RU2186106C1 |
| STRAIN OF T2/S-6E11 HYBRID CELLS OF ANIMAL MUS MUSCULUS L. PRODUCING MONOCLONAL ANTIBODIES RAISED TO CORONAVIRUS AS PATHOGEN OF SEVERE ACUTE RESPIRATORY SYNDROME | 2005 |
|
RU2291195C1 |
| METHOD FOR PRODUCING A SET OF FLUORESCENT RICKETTSIAL AND QUERY FEVER DIAGNOSTICUMS AND USE THEREOF FOR SEROLOGICAL DIAGNOSIS OF RICKETTSIOSIS AND QUERY FEVER, A SEROLOGICAL DIAGNOSTIC TECHNIQUE | 2019 |
|
RU2728340C1 |
| METHOD FOR PRODUCING A FLUORESCENT IMMUNOGLOBULIN DIAGNOSTIC AGENT FOR DETECTING PATHOGENS OF RICKETTSIOSES AND COXIELLOSES, FLUORESCENT IMMUNOGLOBULIN DIAGNOSTIC AGENT AND APPLICATION THEREOF | 2021 |
|
RU2769578C1 |
| STRAIN COD OF SEVERE ACUTE RESPIRATORY SYNDROME VIRUS OF GENUS CORONAVIRUS DESIGNATED FOR DEVELOPMENT OF AGENTS AND METHODS FOR BIOLOGICAL PROTECTION | 2003 |
|
RU2263144C2 |
| DIAGNOSTIC TECHNIQUE FOR RICKETTSIAL DISEASES GROUP OF SPOTTED FEVER, IMMUNOASSAY DIAGNOSTIC TEST SYSTEM FOR IMPLEMENTATION THEREOF | 2019 |
|
RU2726484C1 |
| STRAIN OF HYBRID ANIMAL CELLS Mus MUSCULUS 1F1 - PRODUCER OF A MONOCLONAL ANTIBODY AGAINST THE NUCLEOCAPSID PROTEIN N OF THE SARS-CoV-2 VIRUS | 2021 |
|
RU2769817C1 |
| PSEUDOTUBERCULOUS ERYTHROCYTIC MONOCLONAL DIAGNOSTICUM | 2008 |
|
RU2377308C1 |
