FIELD: biotechnology, hybridoma technology.
SUBSTANCE: hybridoma T2/S-6E11 is prepared by fusion of murine myeloma strain Sp-2/0 and murine BALB/c splenocytes immunized with the TOPC virus, strain CoD, purified preparation inactivated with concentrate formaldehyde solution and by using polyethylene glycol-1000 Da and the following cloning by method of limited dilution. Prepared hybridoma T2/S-6E11 produces monoclonal antibodies (MCAb) to epitopes of the abovementioned pathogen. Specificity of prepared MCAb is shown by absence of cross-reactions in IFA-test with Hantaan and Ebol viruses and with noninfected substrates accumulated by TOPC virus. IFA-test system based on MCAb provides carrying out the specific detection of TOPC virus in analyzed samples. The sensitivity of this test-system based on MCAb is estimated to be 2.5 x 103 plaque-forming units (virus) in cubic centimeter (PFU/cm3). Using the invention in immunological researches in creature of diagnosticum used for detection of TOPC virus in samples provides enhancing the specificity of analysis in detection of coronaviruses.
EFFECT: valuable properties of strain.
3 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF HYBRID CELLS E4/N-6 G5 OF ANIMAL MUS MUSCULUS L PRODUCING MONOCLONAL ANTIBODIES RAISED TO EBOL'S VIRUS | 2001 |
|
RU2186106C1 |
| STRAIN OF HYBRID CELLS M/N-10G9 OF ANIMAL MUS MUSCULUS L PRODUCING MONOCLONAL ANTIBODIES RAISED TO MARBURG VIRUS | 2001 |
|
RU2186107C1 |
| STRAIN OF MAMMALIAN HYBRID CELL C3/S-3E5 OF MUS MUSCULUS L. PRODUCING MONOCLONAL ANTIBODY TO BERNET'S COXIELLAS | 2003 |
|
RU2257414C1 |
| STRAIN OF HYBRID CELLS R1/S-5A6 OF ANIMALS MUS MUSCULUS L PRODUCING MONOCLONAL ANTIBODY RAISED TO RICKETTSIA PROWAZEKII | 2001 |
|
RU2198922C2 |
| STRAIN COD OF SEVERE ACUTE RESPIRATORY SYNDROME VIRUS OF GENUS CORONAVIRUS DESIGNATED FOR DEVELOPMENT OF AGENTS AND METHODS FOR BIOLOGICAL PROTECTION | 2003 |
|
RU2263144C2 |
| STRAIN OF HYBRID ANIMAL CELLS Mus MUSCULUS 1F1 - PRODUCER OF A MONOCLONAL ANTIBODY AGAINST THE NUCLEOCAPSID PROTEIN N OF THE SARS-CoV-2 VIRUS | 2021 |
|
RU2769817C1 |
| STRAIN AS25 OF CONSTANT HYBRIDOMA CELL LINE OF MOUSE Mus musculus-PRODUCER OF MONOCLONAL ANTIBODIES TO ANTIGEN H3 OF EQUINE INFLUENZA VIRUS | 2013 |
|
RU2549713C1 |
| STRAIN OF HYBRID ANIMAL CELLS MUS MUSCULUS 2E1B5 - PRODUCER OF A MONOCLONAL ANTIBODY AGAINST THE RECEPROT-BINDING DOMAIN OF PROTEIN S OF THE SARS-CoV-2 VIRUS | 2021 |
|
RU2771288C1 |
| ANIMAL MUS MUSCULUS HYBRID CELL F3H10 CLONE L-PRODUCER OF MONOCLONAL DIPHTHERIA TOXIN ANTIBODIES | 2009 |
|
RU2407795C1 |
| ANIMAL MUS MUSCULUS HYBRID CELL G10B6 CLONE L-PRODUCER OF MONOCLONAL DIPHTHERIA TOXIN ANTIBODIES | 2009 |
|
RU2401303C1 |
