FIELD: veterinary science.
SUBSTANCE: the method deals with growing bacterial mass of Salmonella followed by its deposition, treating with 12%-sodium chloride solution, autoclaving at 121 C for 20 min, supplementing detergent, sedimentation due to centrifuging at 8-9 thousand rot./min for 30-35 min followed by applying supernatant (lipopolysaccharide-protein complex) to sensitize ram's formalinized erythrocytes. The method is considered to be economical, available and enables to obtain active and specific erythrocytic diagnostic kit.
EFFECT: higher efficiency.
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Authors
Dates
2005-07-27—Published
2003-08-25—Filed