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2 259 401

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IPC

(21) (22)

Application

2004112594/13, 2004-04-27

(24)

Start date

2004-04-27

(22)

Actual filing date

2004-04-27

(45)

Published

2005-08-27

(72)

Inventor

Shajkhaev G.O.

(73)

Holder

Obshchestvo S Ogranichennoj Otvetstvennost'JuObshchestvo S Ogranichennoj Otvetstvennost'Ju Izogen"

DRY MIXTURE OF REAGENTS FOR POLYMERASE CHAIN REACTION AND METHOD FOR CARRYING OUT PCR-ANALYSIS Russian patent published in 2005 - IPC

Abstract RU 2259401 C1

FIELD: molecular biology, biotechnology.

SUBSTANCE: dry mixture of reagents for polymerase chain reaction (PCR) is prepared by lyophilic drying an aqueous solution containing DNA polymerase, deoxyribonucleoside triphosphates, buffer components, dye for electrophoresis, D-glucose, inulin and D-mannitol. For carrying out PCR-analysis the indicated mixture is dissolved in buffer solution containing magnesium ions, specific primers are added to the solution and DNA to be analyzed and amplification of nucleic acid is carried out under condition of polymerase chain reaction. Applying the invention allows retaining the reaction activity of components for polymerase chain reaction in prolonged storage and simplifies carrying out PCR-analysis. Invention can be used in veterinary science, medicine and food industry.

EFFECT: improved method for carrying out PCR-analysis.

9 dwg, 7 ex

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RU 2 259 401 C1

Authors

Shajkhaev G.O.

Dates

2005-08-27—Published

2004-04-27—Filed