METHOD FOR REAL-TIME DETECTION OF NUCLEIC ACIDS BY POLYMERASE CHAIN REACTION AND DEVICE FOR IMPLEMENTATION THEREOF Russian patent published in 2010 - IPC C12P19/00 C12Q1/68 

FIELD: medicine.

SUBSTANCE: there is designed a microchip on a thermal substrate with thermal conduction exceeding 1 Wt/cm·K and thermal diffusion factor exceeding 0.6 cm²/s. Reaction zones on the surface of the microchip are separated from the thermal substrate by a layer of surface-passivating material. The samples are isolated from the atmosphere by a layer of liquid not mixed with water. The liquid is kept by a peripheral barrier. The relation of total thermal weight of the microchip with the introduced samples and the isolating layer of liquid to thermal conduction of the substrate does not exceed 0.04 seconds.

EFFECT: invention allows increasing the thermal cycling rate, eliminating the uncontrolled displacement of the sample with respect to the reaction zone and eliminating inhibition of the reaction.

27 cl, 5 dwg, 3 ex