FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology and concerns a method for separation of lipopolysaccharides of gram-negative bacteria. The method is implemented by gel-chromatography of lipopolysaccharides on a Sephadex G-200 column in an aqueous 0.05M triethylammonium acetate buffer, pH 4.7 containing 10% of n-propanol.
EFFECT: presented method enables producing lipopolysaccharide fractions containing no chromatographic buffer ingredients and being subsequently used in immunopharmacology.
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