FIELD: chemistry.
SUBSTANCE: group of inventions relates to a target-discriminating probe (TD-probe), method of its constructing and methods of detecting a nucleic acid target-sequence with its application. The TD-probe is hybridised with the nucleic acid target-sequence both via a 5'-terminal second site of hybridisation and a 3'-terminal first site of hybridisation. When the TD-probe is hybridised with the nucleic acid sequence which is not a target, then the 5'-terminal second site of hybridisation and a separating site both are not hybridised with the nucleic acid sequence, which is not a target, in such a way that both the sites form a single chain as a result of a low value of its Ts.
EFFECT: claimed inventions make it possible to increase the specificity of hybridisation and can be used in the area of clinical diagnostics and genetic research.
39 cl, 14 dwg, 9 ex
