FIELD: medicine.
SUBSTANCE: method involves cultivation of an obligate methanol-assimilating bacterium Methylophilus methylotrophus or Methylobacillus glycogens in a fluid medium with the bacterium secreting an end protein from a bacterial cell where said bacterium has a DNA structure containing a promoter sequence functioning in the methanol-assimilating bacterium, a nucleotide sequence coding a polypeptide containing a signal sequence which functions in the methanol-assimilating bacterium, and a sequence of the end protein functionally connected with the promoter sequence.
EFFECT: method allows producing the protein effectively by means of extracellular secretion, difficult-to-produce by means of secretory production with application of Escherichia coli bacteria.
5 cl, 7 ex
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| BACTERIUM OF GENUS BACILLUS PRODUCING L-AMINO ACID AND METHOD FOR PREPARING L-AMINO ACID | 2002 |
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| PREPHENATE DEHYDROTASE-CHORISMATMUTASE AND DNA FRAGMENT ENCODING PREPHENATE DEHYDROTASE-CHORISMATMUTASE FROM METHYLOPHILUS METHYLOTROPUS BACTERIUM | 2003 |
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