METHOD FOR DETECTING INDEX OF LYMPHOCYTIC STIMULATION FOR EVALUATING IMMUNE STATE IN MINKS Russian patent published in 2005 - IPC

FIELD: veterinary science.

SUBSTANCE: the suggested method deals with isolating lymphocytes out of peripheral blood and lymphoid organs to achieve their concentration up to working concentration followed by reaction of blast-transformation with mitogen as phytohemagglutinin, incubation of lymphocytes and registration of reaction results by radiometric method: concentration of lymphocytes should achieve working concentration value of 10-15x10⁶ cells/ml, incubation of lymphocytes should be performed for 48-54 h at 37.5-40.0 C, index of lymphocytic stimulation should be calculated by the following formula:index of stimulation = (average amount of impulses/min in experiment) / average amount of impulses/min in control) The method provides increased efficiency in evaluating immune state in minks due to increased sensitivity in reaction of blast-transformation.

EFFECT: higher accuracy and efficiency of detection.

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