METHOD OF CRYOPRESERVATION OF UMBILICAL BLOOD Russian patent published in 2005 - IPC

FIELD: cryobiology.

SUBSTANCE: method, which can be utilized when performing allotransplantation in oncology and hematology, is characterized by that to freshly collected umbilical blood equal volume of polyglucin is added, after which mixture is stirred, and kept for 50-60 min until clear boundary appears. Separated supernatant is then centrifuged to obtain precipitate, to which equal volume of 10-12% dimethylsulfoxide dissolved in polyglucin is added at continuous stirring. Mixture is placed in thermostatically controlled space while lowering temperature at a rate of 1-2°C/min for 1-2 h, after which kept at extremely low temperatures.

EFFECT: maximally preserved vitality of nucleus-bearing cells and thereby enabled transplantations at many severe hematological diseases, including oncological diseases.