FIELD: medicine.
SUBSTANCE: method for stem cell cryopreservation involves preparing a stem cell suspension and adding a cryoprotector solution to the above suspension with constant stirring, which is followed by cryopreservation, wherein the cyoprotector added to the suspension is 50% DMSO in rheopolyglucin to the final DMSO concentration of 10% in the prepared suspension. The group of inventions also concerns a combination cryoprotector for stem cell cryopreservation, containing 50% DMSO in rheopolyglucin.
EFFECT: creating the cryoprotector with a lower molecular weight and lower DMSO concentration for improving post-thawing cell preservation.
2 cl, 1 ex, 5 tbl
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR REDUCING TOXICITY OF CRYOPRESERVATION SOLUTION BASED ON DIMETHYL SULFOXIDE AFTER THAWING OF HEMATOPOIETIC STEM CELLS | 2020 |
|
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Authors
Dates
2015-09-20—Published
2014-12-24—Filed