DETECTION OF NUCLEOTIDE VARIATIONS IN NUCLEIC ACID SEQUENCE-TARGETS IN THE ANALYSIS WITH THE CLEAVAGE AND EXTENSION OF THE PROBING AND TAGGING OLIGONUCLEOTIDE (PTO) Russian patent published in 2017 - IPC C12Q1/68 C12N15/11 

FIELD: biotechnology.

SUBSTANCE: invention relates to a method for detecting nucleotide variation by analysis with cleavage and extension of probing and tagging oligonucleotide (PTOCE analysis), and can be used in medicine. The invention provides the use of PTO-NV, comprising a recognition site of nucleotide variation is located at the 5'-end portion of the 3'-terminal portion of the target recognizer for PTO selectivity in respect of a particular nucleotide variation.

EFFECT: increased efficiency of application.

39 cl, 35 dwg, 18 tbl, 10 ex