METHOD OF TREATING OPERATIONAL BIOPSY AND SECTIONAL MATERIAL FOR MICROSCOPIC EXAMINATION Russian patent published in 2005 - IPC

FIELD: pathohistology.

SUBSTANCE: samples are placed onto thread-like substrate with 0.5-cm intervals to form a bunch 10-12 cm long, which is loosely placed in container. The latter is successively passed through a series of vessels containing corresponding solutions, while being rotated around its axis to favor uniform treatment of material. Time and conditions are set in accordance with selected procedure. Treatment is commenced by dipping material into formalin at 34-37°C for 2 h followed by washing with running water during 4 h. Material is then dehydrated with 96% alcohol by way of passing container through five vessels: in the first one material is dehydrated for 5 h, in the following three for 3 h in each, and in the fifth vessel for 5 h. Tannage is carried out in alcohol-xylene solution for 1 h and then in xylene for 1 h, after which material is flooded with paraffin at 36°C for 1 h and at 56°C also for 1 h. Finally material is cut into block, which are pasted on wooden supports, cut on microtome, cuts are stained and subjected to microscopic examination.

EFFECT: reduced treatment time.

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