METHOD FOR PREPARING BIOLOGICAL TISSUE SAMPLES FOR HISTOPATHOLOGICAL EXAMINATIONS Russian patent published in 2013 - IPC G01N1/28 

FIELD: medicine.

SUBSTANCE: method for preparing biological tissue sample for histopathological examination wherein the biological tissue samples are sequentially processed in a fixative solution which is presented by 10% formalin buffered by sodium phosphates; the intermediate processing takes place at room temperature. Then, the biological tissue samples are washed with water. That is followed by dehydration in more than one portions of isopropanol. The samples are cleared in two portions of the intermediates containing isopropanol and mineral oil with the first portion of the intermediate containing five portions of isopropanol and one portion of mineral oil, and with the second portion containing two portions of isopropanol and one portion of mineral oil. The intermediate processing is followed by immersing the biological tissue samples into mineral oil for clearing. The biological tissue samples are impregnated with melted paraffin added with 5% of bee wax, 0.8% of dimethyl sulphoxide and 0.5% of butyl rubber.

EFFECT: reducing labour content and time for preparing the high-quality section of the histopathological examinations with the either laboratory facilities: manual and automatic lines, in any tissue processors due to the high quality biological tissue samples having been prepared.

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