FIELD: molecular biology, in particular detection of biological objects.
SUBSTANCE: claimed method includes addition of uracyl-DNA-glycosylase in mixture for reverse transcriptase reaction and carrying out of reverse transcriptase and polymerase chain reaction in single reaction volume.
EFFECT: high effective protection of RT-PCR from contamination by amplification products.
1 tbl, 2 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTION OF MICROORGANISMS USING ISOTHERMIC AMPLIFICATION REACTION - EPA (EXONUCLEASE-POLYMERASE AMPLIFICATION) | 2003 |
|
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| METHOD FOR AMPLIFICATION OF MATRIX NUCLEIC ACID | 2001 |
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| METHOD FOR DETECTION OF MICROORGANISMS USING ISOTHERMIC AMPLIFICATION REACTION - RAMIL (REVERTASE AMPLIFICATION ILLATION) OR RNASE H-DEPENDENT AMPLIFICATION ILLATION | 2003 |
|
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| DOSAGE FORM FOR AMPLIFICATION OF NUCLEIC ACIDS | 2020 |
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| METHOD FOR DETERMINATION OF NUCLEIC ACID MOLECULE SEQUENCE CHARACTERISTICS VIA DETECTION AND CHARACTERIZATION OF SAID SEQUENCE VARIATIONS | 1998 |
|
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| METHOD FOR DETECTION OF NUCLEIC ACID-TARGET IN SAMPLE | 2001 |
|
RU2265058C2 |
| METHOD OF ALLEL-SPECIFIC PCR FOR CATTLE GENETIC TYPING BY KAPPA-CASEIN A AND B GENE ALLELS | 2006 |
|
RU2337141C2 |
| DRY MIXTURE OF REAGENTS FOR POLYMERASE CHAIN REACTION AND METHOD FOR CARRYING OUT PCR-ANALYSIS | 2004 |
|
RU2259401C1 |
| METHOD FOR CARRYING OUT POLYMERASE CHAIN REACTION (PCR) | 2005 |
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RU2299240C2 |
| METHOD FOR PRODUCTION OF TYPE 2 PIG CIRCOVIRUS CAPSID PROTEIN AND PHARMACEUTICAL COMPOSITION CONTAINING IT | 2015 |
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RU2701816C1 |
