METHOD FOR DETECTION OF MICROORGANISMS AND SET FOR ITS REALIZATION Russian patent published in 2005 - IPC

FIELD: microbiology.

SUBSTANCE: invention relates to a method for detecting microorganisms in any sample, in particular, in field of medicine, biotechnology, food industry and so on. Method involves the following stages: a) joining bacteriophages and/or bacteriophage proteins with a carrier; b) a carried joined with bacteriophages and/or bacteriophage proteins is incubated with sample, sample and microorganisms in the sample not binding with bacteriophages and/or bacteriophage proteins can be removed followed by addition of substances penetrating or destroying bacterial membranes; c) microorganisms in the sample bound with bacteriophages and/or bacteriophage proteins are detected and bound microorganisms are not subjected for the culturing stage. The set used for realization of the method comprises a carrier with immobilized phages or bacteriophages proteins wherein the latter are bound by direct binding with a carrier or by direct binding with polypeptide immobilized on the carrier, and reagents required for analysis in detection of bound microorganisms. The set comprises additionally solutions for washing out and/or substances penetrating or destroying microorganism membranes. Invention provides the possibility for rapid and sensitive detection of microorganisms.

EFFECT: improved method for detection.

14 cl, 7 dwg, 5 ex