METHOD FOR PRODUCTION OF PROBIOTIC PREPARATION Russian patent published in 2005 - IPC

FIELD: biotechnology, in particular production of probiotic bacterial preparations for disease prophylaxis in animals.

SUBSTANCE: Lactobacillus acidophilus Scav.B-4625, Rhuminococcus albus Kr, Bacillus subtilis B-8130 from Bacillus subtilis microorganisms are separately cultivated under conditions of deep cultivation, wherein Lactobacillus acidophilus Scav.B-4625 and Rhuminococcus albus Kr are cultivated in liquid broth, containing rumen liquid, sunflower oilcake, meat-and-peptone broth, sodium chloride, magnesium sulfate, disubstituted potassium phosphate, ammonium sulfate, calcium carbonate and water in specific component ratio for 4-6 days to obtain titer for each microorganism of 1.10⁸ - 3.10⁸. Strain Bacillus subtilis B-8130 is cultivated in liquid broth, containing yeast, molasses, sunflower oilcake, gluten, magnesium sulfate, disubstituted potassium phosphate, calcium carbonate and water in specific component ratio for 4-6 days at 37-45°C, adjusting pH up to 7.2-7.4 to obtain titer for Bacillus subtilis B-8130 of 1.10⁸ - 2.10⁸. Then microorganism cultural liquids are blended in the next ratio (mass %): Lactobacillus acidophilus Scav.B-4625 and Rhuminococcus albus Kr cultural liquids 45-55; Bacillus subtilis B-8130 cultural liquid 45-55.

EFFECT: preparation for animal and poultry prophylaxis and treatment having antimicrobial protection and stimulating activity; increased conversion of feed with high cellulose content; enhanced assortment of medicated and preventive natural preparations.

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