FIELD: biotechnology, microbiology, genetic engineering.
SUBSTANCE: invention relates to methods for preparing lactic acid. Lactic acid is prepared by culturing the recombinant strain of yeast of genus Schizosaccharomyces as a producer of lactic acid comprising at least one foreign lactate dehydrogenase gene. The recombinant strain of yeast Schizosaccharomyces pombe VKPM Y-3127 is prepared by using the strain Schizosaccharomyces pombe VKPM Y-285 as a recipient and transformation of the strain is carried out with plasmid DNA carrying IdhA gene from fungus Rhizopus oryzae. Invention provides carrying out synthesis of lactic acid at low pH values and show capacity for producing lactic acid at pH 4.0 up to the concentration of lactic acid in medium 80-100 g/l.
EFFECT: improved preparing method.
3 cl, 5 dwg, 2 tbl, 4 ex
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