FIELD: biotechnology, microbiology, genetic engineering.
SUBSTANCE: invention relates to methods for preparing lactic acid. Lactic acid is prepared by culturing the recombinant strain of yeast of genus Schizosaccharomyces as a producer of lactic acid comprising at least one foreign lactate dehydrogenase gene. The recombinant strain of yeast Schizosaccharomyces pombe VKPM Y-3127 is prepared by using the strain Schizosaccharomyces pombe VKPM Y-285 as a recipient and transformation of the strain is carried out with plasmid DNA carrying IdhA gene from fungus Rhizopus oryzae. Invention provides carrying out synthesis of lactic acid at low pH values and show capacity for producing lactic acid at pH 4.0 up to the concentration of lactic acid in medium 80-100 g/l.
EFFECT: improved preparing method.
3 cl, 5 dwg, 2 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SCHIZOSACCHAROMYCES POMBE STRAIN - LACTIC ACID PRODUCER | 2016 |
|
RU2650669C1 |
| YEAST TRANSFORMANT SCHIZOSACCHAROMYCES POMBE, PRODUCING LACTIC ACID (VERSIONS) METHOD FOR PRODUCTION THEREOF (VERSIONS), METHOD FOR LACTIC ACID MICROBIOLOGICAL SYNTHESIS USING THIS TRANSFORMANT | 2015 |
|
RU2614233C1 |
| STRAIN OF YEAST SCHIZOSACCHAROMYCES POMBE, PRODUCING L-LACTIC ACID, CONTAINING GENES OF THREE DIFFERENT HETEROLOGOUS LACTATE DEHYDROGENASES IN CHROMOSOME | 2020 |
|
RU2752896C1 |
| RECOMBINANT STRAIN OF YEASTS Schizosaccharomyces pombe - PRODUCENT OF LACTIC ACID | 2013 |
|
RU2539092C1 |
| METHOD FOR MODIFICATION OF YEAST SCHIZOSACCHAROMYCES POMBE BY MEANS OF THE CRE-LOX SYSTEM OF BACTERIOPHAGE P1, TRANSFORMANT THAT IS OBTAINED IN SUCH A METHOD | 2016 |
|
RU2652877C9 |
| YEAST STRAIN Yarrowia lipolytica - PRODUCER OF CELL-BOUND LIPASE | 2012 |
|
RU2475532C1 |
| GENETIC CONSTRUCTION FOR OBTAINING STABLE TRANSFORMANTS OF FUNGI OF GENUS Rhizopus | 2009 |
|
RU2439157C2 |
| RECOMBINANT YEAST STRAIN YARROWIA-LIPOLYTICA LIPASE PRODUCER | 2011 |
|
RU2451075C1 |
| YEAST STRAIN Yarrowia lipolytica - LIPASE PRODUCER | 2007 |
|
RU2355754C1 |
| YEASTS OF GENUS YARROWIA, HAVING ABILITY TO ACCUMULATE INTRACELLULARLY FATTY ACID ESTERS, AND METHOD OF MICROBIOLOGICAL SYNTHESIS OF SUCH ESTERS | 2013 |
|
RU2539744C1 |
