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(19)

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2 752 896

(13)

(51)

IPC

C12N1/19(2006-01-01)

C12N9/04(2006-01-01)

C12N9/88(2006-01-01)

C12N15/53(2006-01-01)

C12N15/60(2006-01-01)

C12N15/81(2006-01-01)

C12P7/56(2006-01-01)

(21) (22)

Application

2020139937, 2020-12-04

(24)

Start date

2020-12-04

(22)

Actual filing date

2020-12-04

(45)

Published

2021-08-11

(72)

Inventor

Anisimova Ekaterina OlegovnaTarutina Marina GennadevnaShutov Artem VladimirovichFedorov Aleksandr SergeevichSineokij Sergej Pavlovich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Nauchno-Issledovatelskij Institut Genetiki I Selektsii Promyshlennykh Mikroorganizmov Natsionalnogo Issledovatelskogo Tsentra Institut" Gosniigenetika)

STRAIN OF YEAST SCHIZOSACCHAROMYCES POMBE, PRODUCING L-LACTIC ACID, CONTAINING GENES OF THREE DIFFERENT HETEROLOGOUS LACTATE DEHYDROGENASES IN CHROMOSOME Russian patent published in 2021 - IPC C12N1/19 C12N9/04 C12N9/88 C12N15/53 C12N15/60 C12N15/81 C12P7/56

Abstract RU 2752896 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. A recombinant strain of Schizosaccharomyces pombe VKPM Y-4822, which is a producer of L-lactic acid, is proposed. The strain has a deleted pyruvate decarboxylase PDC1 gene and carries lactate dehydrogenase genes from Lactobacillus pentosus, Lactobacillus acidophilus and Lactobacillus helveticus in the chromosomal DNA. The strain produces L-lactic acid in an amount of 125 g/l for 72 hours of cultivation in a fermenter under aerobic conditions.

EFFECT: invention makes it possible to expand the arsenal of recombinant microorganisms that produce L-lactic acid.

1 cl, 2 dwg, 3 ex

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RU 2 752 896 C1

Authors

Anisimova Ekaterina Olegovna

Tarutina Marina Gennadevna

Shutov Artem Vladimirovich

Fedorov Aleksandr Sergeevich

Sineokij Sergej Pavlovich

Dates

2021-08-11—Published

2020-12-04—Filed