FIELD: molecular biology, biochemistry, microbiology, medicine.
SUBSTANCE: method involves the simultaneous amplification of DNA and RNA targets and E. coli plasmid fragment or E. coli 16S-RNA as universal standard. For amplification two pairs of primers with the identical annealing temperature are used being primers of the first pair are specific to the target nucleotide sequence and primers of the second pairs of primers - to nucleotide sequence of the internal standard. A number of target copies are determined by data of electrophoretic separation of target amplicons and the universal internal standard. Using the invention allows carrying out the determination of amount of copies of DNA and RNA targets.
EFFECT: improved assay method.
4 dwg, 1 tbl
