FIELD: biotechnology.
SUBSTANCE: methods of obtaining a large fragment of Bst polymerase in the form of a recombinant protein in E. coli cells with a high yield of the enzyme in soluble form are described. The methods include the steps of synthesizing a nucleotide sequence, obtaining an expression construct, expressing a large fragment of Bst polymerase, and preparing a large fragment of Bst polymerase.
EFFECT: development of options for an effective method of obtaining a large fragment of Bst polymerase in a soluble form with high yield.
5 cl, 6 dwg, 5 tbl, 5 ex
