FIELD: biotechnology, biochemistry, enzymology, genetic engineering.
SUBSTANCE: invention relates to preparing new strain used for isolation of the new restriction endonuclease Bis I that can be used in detection of the modified DNA. The strain Bacillus subtilis 230 isolated from soil as result of search of producers of restriction endonucleases provides preparing the restriction endonuclease Bis I recognizing and cleaving both chain of DNA nucleotide sequence comprising at least one C5-methylcytosine base in the recognition site 5'-GCNGC-3'.
EFFECT: valuable properties of strain, new enzyme.
1 tbl, 3 dwg, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| GLACIAL ICE BACTERIUM MICROORGANISM STRAIN AS PRODUCER OF SITE-SPECIFIC ENDONUCLEASE GLU I | 2006 |
|
RU2322492C1 |
| MICROORGANISM STRAIN BACILLUS SIMPLEX AS PRODUCER OF SITE-SPECIFIC ENDONUCLEASE BlsI | 2006 |
|
RU2322494C1 |
| BACTERIA STRAIN Planomicrobium koreense 78K-PRODUCENT OF SITE-SPECIFIC ENDONUCLEASE PkrI | 2011 |
|
RU2475534C1 |
| STRAIN OF BACTERIA MICROCOCCUS LUTEUS 805 - PRODUCER OF SITE-SPECIFIC METHYL-DEPENDEDN ENDONUCLEASE MluVI | 2016 |
|
RU2614262C1 |
| Arthrobacter oxydans BACTERIA STRAIN - AoxI SITE-SPECIFIC ENDONUCLEASE PRODUCER | 2009 |
|
RU2399663C1 |
| BACTERIA STRAIN Microbacterium testaceum 17B-PRODUCENT OF SITE-SPECIFIC ENDONUCLEASE MteI | 2011 |
|
RU2475533C1 |
| STRAIN OF BACTERIUM Paracoccus carotinifaciens 3K - PRODUCER OF SITE-SPECIFIC ENDONUCLEASE PcsI | 2008 |
|
RU2377294C1 |
| STRAIN OF BACTERIA Kocuria rasea-PRODUCER OF SITE-SPECIFIC ENDONUCLEASES KroI | 2009 |
|
RU2394099C1 |
| Arthrobacter luteus B BACTERIA STRAIN-PRODUCER OF SITE-SPECIFIC Alu BI ENDONUCLEASE | 2007 |
|
RU2340670C1 |
| STRAIN OF BACTERIUM Glacial ice bacterium I AS PRODUCER OF RESTRICTION ENDONUCLEASE Gla I | 2005 |
|
RU2287012C1 |
