FIELD: biotechnology.
SUBSTANCE: strain Micrococcus luteus, deposited under registration number B-12410 in The All-Russia Collection of Industrial Microorganisms of GosNIIGenetika FSUE is the producer of the site-specific methyl-dependent endonuclease MluVI. The invention provides a new MD-endonuclease enzyme which recognizes and cleaves both strands of the DNA sequence 5'-GCGC^NGCGC-3'/3'-CGCGN^CGCG-5' before the central nucleotide (N) with formation of single-nucleotide 5'-protruding ends in the presence in a given sequence of at least six C5-methylized cytosines and can be used for large-site-specific hydrolysis of DNA containing C5-methyl-cytosine bases.
EFFECT: increase the efficiency of strain application.
2 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
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| RECOMBINANT BACTERIAL STRAIN Escherichia coli N42 (pElmI) - PRODUCER OF METHYL-DEPENDANT SITE-SPECIFIC ENDONUCLEASE ElmI | 2015 |
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RU2597987C1 |
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| MICROORGANISM STRAIN BACILLUS SIMPLEX AS PRODUCER OF SITE-SPECIFIC ENDONUCLEASE BlsI | 2006 |
|
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| METHOD FOR SITE-SPECIFIC DNA HYDROLYSIS | 2015 |
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RU2597984C1 |
