FIELD: biotechnology, genetic engineering, biochemistry.
SUBSTANCE: strain of microorganism Glacial ice bacterium is isolated from soil and can be used for a preparing site-specific endonuclease that recognizes and cleaves both chain of methylated nucleotide sequence in DNA: 5'-G(m5C)↓NG(m5C)-3'. Use of the invention provides preparing the novel site-specific endonuclease showing specificity to a methylated sequence in DNA that can be used in DNA analysis.
EFFECT: valuable biological properties of endonuclease.
1 tbl, 3 dwg, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| BACTERIA STRAIN Microbacterium testaceum 17B-PRODUCENT OF SITE-SPECIFIC ENDONUCLEASE MteI | 2011 |
|
RU2475533C1 |
| STRAIN OF BACTERIUM Paracoccus carotinifaciens 3K - PRODUCER OF SITE-SPECIFIC ENDONUCLEASE PcsI | 2008 |
|
RU2377294C1 |
| BACTERIA STRAIN Planomicrobium koreense 78K-PRODUCENT OF SITE-SPECIFIC ENDONUCLEASE PkrI | 2011 |
|
RU2475534C1 |
| STRAIN OF BACTERIA Kocuria rasea-PRODUCER OF SITE-SPECIFIC ENDONUCLEASES KroI | 2009 |
|
RU2394099C1 |
| Arthrobacter oxydans BACTERIA STRAIN - AoxI SITE-SPECIFIC ENDONUCLEASE PRODUCER | 2009 |
|
RU2399663C1 |
| STRAIN OF BACTERIA MICROCOCCUS LUTEUS 805 - PRODUCER OF SITE-SPECIFIC METHYL-DEPENDEDN ENDONUCLEASE MluVI | 2016 |
|
RU2614262C1 |
| MICROORGANISM STRAIN BACILLUS SIMPLEX AS PRODUCER OF SITE-SPECIFIC ENDONUCLEASE BlsI | 2006 |
|
RU2322494C1 |
| STRAIN OF BACTERIA Plantibacter flavus 3Kz - PRODUCER OF METHYL-DEPENDENT SITE-SPECIFIC ENDONUCLEASE PfsI | 2015 |
|
RU2593723C1 |
| STRAIN OF BACTERIUM BACILLUS SUBTILIS AS PRODUCER OF RESTRICTION ENDONUCLEASE Bisi | 2004 |
|
RU2270859C1 |
| STRAIN OF BACTERIUM Glacial ice bacterium I AS PRODUCER OF RESTRICTION ENDONUCLEASE Gla I | 2005 |
|
RU2287012C1 |
