FIELD: biotechnology, genetic engineering, biochemistry.
SUBSTANCE: invention proposes the strain Bacillus simplex 23 isolated from soil and providing preparing site-specific endonuclease. This enzyme is able for recognizing and cleaving both chain in nucleotide sequence of DNA comprising at least one C5-methylcytosine base in the recognition site 5'-GCNGC-3' to form 3'-prominent ends. The novel strain can be used for isolation of the novel site-specific endonuclease that can be used for detection and cleavage of methylated sites in DNA.
EFFECT: valuable biological and biochemical properties of strain and endonuclease.
4 dwg, 4 ex
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Authors
Dates
2008-04-20—Published
2006-08-24—Filed