MICROORGANISM STRAIN BACILLUS SIMPLEX AS PRODUCER OF SITE-SPECIFIC ENDONUCLEASE BlsI Russian patent published in 2008 - IPC C12N1/20 C12R1/07 

Abstract RU 2322494 C1

FIELD: biotechnology, genetic engineering, biochemistry.

SUBSTANCE: invention proposes the strain Bacillus simplex 23 isolated from soil and providing preparing site-specific endonuclease. This enzyme is able for recognizing and cleaving both chain in nucleotide sequence of DNA comprising at least one C5-methylcytosine base in the recognition site 5'-GCNGC-3' to form 3'-prominent ends. The novel strain can be used for isolation of the novel site-specific endonuclease that can be used for detection and cleavage of methylated sites in DNA.

EFFECT: valuable biological and biochemical properties of strain and endonuclease.

4 dwg, 4 ex

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RU 2 322 494 C1

Authors

Chernukhin Valerij Alekseevich

Tomilova Julija Ehduardovna

Chmuzh Elena Vasil'Evna

Sokolova Ol'Ga Olegovna

Dedkov Vladimir Sergeevich

Degtjarev Sergej Kharitonovich

Dates

2008-04-20Published

2006-08-24Filed