FIELD: biotechnologies.
SUBSTANCE: bacteria strain Planomicrobium koreense 78k, is extracted from soils and provides for production of site-specific endonuclease PkrI, which recognises and splits both chains of nucleotide sequence of DNA, which contains three or four C5-methylcytosine bases in recognition site 5'-GCNAGC-3' with formation of a single-nucleotide 3'-protruding end. The strain is deposited in the Russian National Collection of Industrial Microorganisms FGUP GosNIIgenetika under the number B-10627. The provided strain may be used for extraction of the new site-specific endonuclease PkrI, which may be applied in detection and splitting of methylated DNA sections.
EFFECT: production of site-specific endonuclease PkrI.
3 dwg, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF BACTERIUM Paracoccus carotinifaciens 3K - PRODUCER OF SITE-SPECIFIC ENDONUCLEASE PcsI | 2008 |
|
RU2377294C1 |
| BACTERIA STRAIN Microbacterium testaceum 17B-PRODUCENT OF SITE-SPECIFIC ENDONUCLEASE MteI | 2011 |
|
RU2475533C1 |
| Arthrobacter oxydans BACTERIA STRAIN - AoxI SITE-SPECIFIC ENDONUCLEASE PRODUCER | 2009 |
|
RU2399663C1 |
| STRAIN OF BACTERIA Kocuria rasea-PRODUCER OF SITE-SPECIFIC ENDONUCLEASES KroI | 2009 |
|
RU2394099C1 |
| GLACIAL ICE BACTERIUM MICROORGANISM STRAIN AS PRODUCER OF SITE-SPECIFIC ENDONUCLEASE GLU I | 2006 |
|
RU2322492C1 |
| RECOMBINANT BACTERIAL STRAIN Escherichia coli N42 (pElmI) - PRODUCER OF METHYL-DEPENDANT SITE-SPECIFIC ENDONUCLEASE ElmI | 2015 |
|
RU2597987C1 |
| MICROORGANISM STRAIN BACILLUS SIMPLEX AS PRODUCER OF SITE-SPECIFIC ENDONUCLEASE BlsI | 2006 |
|
RU2322494C1 |
| STRAIN OF BACTERIA MICROCOCCUS LUTEUS 805 - PRODUCER OF SITE-SPECIFIC METHYL-DEPENDEDN ENDONUCLEASE MluVI | 2016 |
|
RU2614262C1 |
| METHOD FOR SITE-SPECIFIC DNA HYDROLYSIS | 2015 |
|
RU2597984C1 |
| STRAIN OF BACTERIUM BACILLUS SUBTILIS AS PRODUCER OF RESTRICTION ENDONUCLEASE Bisi | 2004 |
|
RU2270859C1 |
