FIELD: medicine.
SUBSTANCE: Arthrobacter oxydans 25K bacteria strain is recovered from soil. This strain enables preparation of Aoxl site-specific endonuclease that identifies and splitting both chains of a nucleotide DNA sequences 5'-GGCC-3'/3'-CCGG-5' wherein two central cytosine are methylated in C5 position to form four-nucleotide 5'-overhanging ends. The presented invention allows producing bacterial strain producing site-specific endonuclease which identifies and splits both chains of nucleotide DNA sequences 5'-∧GG (m5C)C-3/3'-C(m5C)GG∧-5'.
EFFECT: higher efficiency of application of the compounds.
2 dwg, 3 ex
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