METHOD FOR QUANTITATIVE EVALUATION OF STIMULATED COLLAGENOGENESIS WITH BIOPLASTIC MATERIALS Russian patent published in 2006 - IPC G01N33/68 C12N5/08 A61K35/36 

FIELD: medicine.

SUBSTANCE: one should place bioplastic material in culture liquid with a culture of dermal fibroblasts to cultivate fibroblasts together with bioplastic material for 4 d, then it is necessary to sample culture liquid and detect the concentration of protein-bound hydroxyproline. One concludes upon the degree of collagenogenesis stimulation with tested bioplastic material by greater or lesser degree of increased concentration of protein-bound hydroxyproline in sampled culture liquid against the control. As a control sample it is useful to apply culture liquid in which one could observe the tested bioplastic material for 4 d but no fibroblasts. The innovation is simple and enables to quantitatively evaluate the stimulation of collagenogenesis with bioplastic materials without applying any laboratory animals.

EFFECT: higher accuracy of evaluation.