FIELD: biology, embryology.
SUBSTANCE: invention relates to a method for controlling oocytes quality and a composition for addition in medium for culturing oocytes. Method for controlling the oocytes quality involves placing at least one oocyte in cultural medium and carrying out culturing. The cultural medium comprises at least one anti-sense oligonucleotide of length 17-30 nucleotides wherein each of them is complementary to mRNA of at least one of the following genes: gene-inductors of apoptosis, such as HRK, FAS, FASL, BAX, Caspase-3, genes of growth factors, such as IGF1, gene-receptors of growth factors, such as IGF1R, genes regulating the dividing embryos rate, such as HLA-E, HLA-F, HLA-G, genes of cellular stress, such as HSF1 and HSF2. The composition added in cultural medium comprises one or more an anti-sense oligonucleotide describes above and acceptable solvent. The advantage of invention involves enhancing the quality and viability of oocytes and enhancing the therapeutic effectiveness in treatment of sterility.
EFFECT: improved controlling method, improved properties of composition.
15 cl, 2 dwg, 10 ex
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