FIELD: medical engineering.
SUBSTANCE: method involves dissolving dry semi-refined polysaccharide in sodium acetate solution with following exposure, treating with phenol mixed with saturated sodium acetate solution, centrifuging, separated upper layer dialysis against calcium chloride solution, depositing with alcohol, centrifuging, separating sediment and its drying. The separated upper layer dialysis product is additionally released from low molecular weight admixtures of molecular weight being below 100 kDa in serological group A case and below 300 kDa in serological group C case by means of ultrafiltration on membranes. Then, 2% saturated sodium acetate solution is added to serological group A polysaccharide separated on membrane and the dissolved polysaccharide is sedimented with four volumes of 96% ethanol cooled to 4-5°C. The group C polysaccharide separated on membrane is subjected to ultracentrifuging at 100000 g during 2.5-3 h. The supernatant layer is separated. Dissolved polysaccharide is sedimented with four volumes of 96% ethanol cooled to 4-5°C.
EFFECT: increased high molecular weight polysaccharide content without drop in output performance.
4 cl, 2 tbl
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