FIELD: medicine.
SUBSTANCE: method consists in the serogroup A strain A 208 N. meningitidis culture growth on the modified Franz nutrient medium that is followed with the cetavlon inactivation of a culture fluid to the final concentration 0.1 % and the centrifugal sedimentation of a microbial mass, the IgAl-protease recovery and purification, and the product lyophilisation. The IgAl-protease recovery is enabled by both fractions, namely cetavlon supernatant and cetavlon sediment. The collected sediment is multiply extracted, the sediment extracts and supernatant are concentrated by an ultrafiltration technique. The prepared concentrates are fractionated by absorption chromatography on a Silochrom-80 sorbent column at temperature +8°C compensated with 0.02 M phosphate salt buffer solution, pH 7.0, with IgAl-protease being produced once washing sorbent with said buffer solution. The invention makes it possible to produce a non-polysaccharide vaccine component for protection against serogroup B meningococcal infection.
EFFECT: invention allows to simplify the method for producing IgAl-protease of N meningitidis due to the substitution of multiphase agent inactivation by a one-phase cetavlon processing, the elimination of a gel-penetration chromatography stage, and to improve ecological safety of the process as a whole.
3 cl, 6 dwg, 2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| NUCLEIC ACID CODING FUNCTIONALLY ACTIVE RECOMBINANT IgAL PROTEASE NEISSERIA MENINGITIDIS OF SEROGROUP B, RECOMBINANT PLASMID DNA CONTAINING NUCLEOTIDE SEQUENCE CODING ACTIVE IgAL PROTEASE, PRODUCER STRAIN CONTAINING PLASMID DNA PRODUCING MATURE FORM OF IgAL PROTEASE, RECOMBINANT Ig PROTEASE NEISSERIA MENINGITIDIS OF SEROGROUP B, METHOD FOR PRODUCING MATURE FORM IgAL PROTEASE SHOWING IMMUNOGENIC AND PROTECTIVE PROPERTIES | 2011 |
|
RU2453599C1 |
| POLYNUCLEOTIDE ENCODING MUTANT RECOMBINANT IgA1 PROTEASE OF Neisseria meningitidis OF SEROGROUP B, RECOMBINANT PLASMID DNA COMPRISING SAID POLYNUCLEOTIDE, HOST CELL CONTAINING SAID PLASMID DNA, RECOMBINANT IgA1 PROTEASE OF Neisseria memingitidis OF SEROGROUP B, METHOD OF PRODUCING MATURE FORM OF IgA1 PROTEASE | 2011 |
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RU2486243C1 |
| RECOMBINANT PROTEIN HAVING PROTECTIVE ACTION ON MENINGOCOCCUS (EMBODIMENTS), POLYNUCLEOTIDE CODING RECOMBINANT PROTEIN, RECOMBINANT PLASMID DNA CONTAINING SAID POLYNUCLEOTIDE, HOST CELL CONTAINING SAID RECOMBINANT PLASMID DNA, METHOD OF PRODUCING RECOMBINANT PROTEIN | 2017 |
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| METHOD FOR PREPARATION OF POLYSACCHARIDO-PROTEIN VACCINE AGAINST NEISSERIA MENINGITIS SEROGROUP B | 0 |
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SU1750689A1 |
| METHOD OF PREPARING MENINGOCOCCUS LIPOPOLYSACCHARIDE OF SEROGROUP B | 1993 |
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