FIELD: medicine.
SUBSTANCE: what is described is nucleic acid coding IgAl protease Neisseria meningitidis of serogroup B, strain H44/76. What is presented is recombinant plasmid DNA containing nucleic acid. What is described is producer strain E.coli BL21 (DE3)/pBIGAPSl containing presented recombinant plasmid DNA. What is presented is a method for producing a mature form of IgAl protease Neisseria meningitidis of serogroup B. What is described is IgAl protease of molecular weight 107.6 kDa and estimated calculated isoelectric point 7.4.
EFFECT: invention allows producing high-purity IgAl protease as an ingredient of a polyvalent vaccine intended for protection of an individual, at first of children, against meningococcal infection and other microorganisms pathogenicity of which is caused by IgAl protease.
5 cl, 4 dwg, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| POLYNUCLEOTIDE ENCODING MUTANT RECOMBINANT IgA1 PROTEASE OF Neisseria meningitidis OF SEROGROUP B, RECOMBINANT PLASMID DNA COMPRISING SAID POLYNUCLEOTIDE, HOST CELL CONTAINING SAID PLASMID DNA, RECOMBINANT IgA1 PROTEASE OF Neisseria memingitidis OF SEROGROUP B, METHOD OF PRODUCING MATURE FORM OF IgA1 PROTEASE | 2011 |
|
RU2486243C1 |
| RECOMBINANT PROTEIN HAVING PROTECTIVE ACTION ON MENINGOCOCCUS (EMBODIMENTS), POLYNUCLEOTIDE CODING RECOMBINANT PROTEIN, RECOMBINANT PLASMID DNA CONTAINING SAID POLYNUCLEOTIDE, HOST CELL CONTAINING SAID RECOMBINANT PLASMID DNA, METHOD OF PRODUCING RECOMBINANT PROTEIN | 2017 |
|
RU2701964C2 |
| METHOD FOR PRODUCING IgAl-PROTEASE FROM SEROGROUP A NEISSERIA MENINGITIDIS AND RELATED IMMUNOGENIC PREPARATION | 2009 |
|
RU2407792C1 |
| NEISSERIA MENINGITIDIS POLYPEPTIDES | 2007 |
|
RU2450019C2 |
| NEISSERIA MENINGITIDIS COMPOSITIONS AND METHODS OF USE THEREOF | 2013 |
|
RU2665841C2 |
| DNA FRAGMENT ENCODING NEISSERIA MEMINGITIDIS STRAIN B:4:P1:15 EXTERNAL MEMBRANE PROTEIN, EXTERNAL MEMBRANE PROTEIN OF NEISSERIA MENINGITIDIS, RECOMBINANT PLASMID DNA PM-6, STRAIN OF BACTERIUM ESCHERICHIA COLI HBM64, METHOD OF PRODUCING PROTEIN OF NEISSERIA MENINGITIDIS EXTERNAL MEMBRANE, VACCINE COMPOSITION | 1991 |
|
RU2132383C1 |
| NEISSERIA MENINGITIDIS COMPOSITIONS AND METHODS FOR USING THEM | 2017 |
|
RU2739504C2 |
| NEISSERIA MENINGITIDIS PEPTIDE FOR THERAPEUTIC AND DIAGNOSTIC APPLICATION | 2001 |
|
RU2313535C2 |
| VACCINE COMPOSITION CONTAINING TRANSFERRIN-BINDING PROTEIN AND HSF FROM GRAM-NEGATIVE BACTERIA | 2003 |
|
RU2359696C2 |
| ANTIGENES NEISSERIA MENINGITIDIS | 1999 |
|
RU2343159C2 |
