FIELD: virology, molecular biology.
SUBSTANCE: invention proposes a method for preparing a stable clone of full-sized cDNA of tick Langat's flavivirus. Method involves multiple infection of cellular line with virus, collection of viral suspension in early culturing phase and isolation of virus. Also, invention proposes the clone prepared by this method, infectious RNA transcribed from the clone, a single dose of immunogenic composition comprising such RNA and a method for inducing the protective immunity. Invention can be used in virology and medicine.
EFFECT: valuable biological and medicinal properties of clones.
22 cl, 11 dwg, 11 tbl, 10 ex
| Title | Year | Author | Number |
|---|---|---|---|
| DNA-CONSTRUCT OF VIABLE CHIMERIC RECOMBINANT FLAVIVIRUS AGAINST TICK-BORNE ENCEPHALITIS VIRUS, METHOD OF PROPHYLAXIS OF INFECTION MAMMAL WITH TICK-BORNE ENCEPHALITIS VIRUS | 1998 |
|
RU2208635C2 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBE FOR JMTV RNA IDENTIFICATION BY PCR WITH HYBRIDISATION-FLUORESCENCE DETECTION | 2023 |
|
RU2818960C1 |
| IMMUNOBIOLOGICAL AGENT FOR THE PREVENTION OF DISEASES CAUSED BY TICK-BORNE ENCEPHALITIS VIRUS BASED ON A RECOMBINANT VIRUS OF THE GENUS FLAVIVIRUS | 2022 |
|
RU2795800C1 |
| PVEAL3-10H10CH PLASMID GENETIC CONSTRUCT, STRAIN OF CHO-K1-10H10CH RECOMBINANT CELL LINE AND 10H10CH CHIMERIC ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS PRODUCED BY THE INDICATED STRAIN OF CHO-K1-10H10CH CELL LINE | 2022 |
|
RU2800471C1 |
| GENE THERAPY OF OPHTHALMIC DISORDERS | 2016 |
|
RU2762747C2 |
| METHODS AND COMPOSITIONS FOR THE TREATMENT OF DISORDERS AND DISEASES RELATED TO RDH12 | 2017 |
|
RU2764920C2 |
| RECOMBINANT STRAIN OF VV-GMCSF-S-Lact VACCINIA VIRUS, WITH ONCOLYTIC ACTIVITY, PRODUCING GRANULOCYTOR-MACROPHAGAL COLONY-STIMULATING HUMAN FACTOR AND SECRETED FORM OF LACTAPTIN ONCOTOXIC PROTEIN | 2016 |
|
RU2621861C1 |
| CHIMERIC FLAVIVIRAL VACCINES | 1998 |
|
RU2209082C2 |
| RECOMBINANT PLASMID DNA pET160-E3-YFV EXPRESSING AN ANALOGUE OF THE THIRD DOMAIN OF THE SURFACE PROTEIN E OF THE YELLOW FEVER VIRUS (YFV) IN THE BACTERIAL SYSTEM OF E. COLI USED TO DETECT SPECIFIC ANTIBODIES OF YFV | 2022 |
|
RU2798563C1 |
| RECESSIVE DIVIDING RETROVIRAL VECTOR (VARIANTS), METHOD FOR PRODUCTION THEREOF (VARIANTS), ISOLATED AND PURIFIED NUCLEIC ACID MOLECULE | 1996 |
|
RU2270250C2 |
