FIELD: biotechnology.
SUBSTANCE: described is a set of oligodeoxyribonucleotide primers and a fluorescent-labelled DNA probe for identifying JMTV RNA by PCR with hybridisation-fluorescent detection. Set contains one pair of oligonucleotides having activity of forward (F) and reverse (R) primers in polymerase chain reaction, as well as a fluorescent-labelled DNA probe (Z), having the following nucleotide sequences SEQ ID NO: 1–3 respectively.
EFFECT: design of a set of oligonucleotide primers and a fluorescent-labelled probe, which can provide high sensitivity of detection solely by JMTV by PCR with hybridisation-fluorescence detection.
1 cl, 6 dwg, 5 tbl, 4 ex
Authors
Dates
2024-05-07—Published
2023-12-04—Filed