SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBE FOR JMTV RNA IDENTIFICATION BY PCR WITH HYBRIDISATION-FLUORESCENCE DETECTION Russian patent published in 2024 - IPC C12Q1/68 C12Q1/6876 C12Q1/6888 

Abstract RU 2818960 C1

FIELD: biotechnology.

SUBSTANCE: described is a set of oligodeoxyribonucleotide primers and a fluorescent-labelled DNA probe for identifying JMTV RNA by PCR with hybridisation-fluorescent detection. Set contains one pair of oligonucleotides having activity of forward (F) and reverse (R) primers in polymerase chain reaction, as well as a fluorescent-labelled DNA probe (Z), having the following nucleotide sequences SEQ ID NO: 1–3 respectively.

EFFECT: design of a set of oligonucleotide primers and a fluorescent-labelled probe, which can provide high sensitivity of detection solely by JMTV by PCR with hybridisation-fluorescence detection.

1 cl, 6 dwg, 5 tbl, 4 ex

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RU 2 818 960 C1

Authors

Lutkovskij Roman Yurevich

Krivosheina Ekaterina Ilinichna

Ternovoj Vladimir Aleksandrovich

Dates

2024-05-07Published

2023-12-04Filed