FIELD: biotechnology.
SUBSTANCE: pVEAL3-10H10ch plasmid, CHO-K1-10H10ch recombinant cell line strain, and 10H10ch chimeric antibody against tick-borne encephalitis virus are described. pVEAL3-10H10ch integrative plasmid vector has been created to ensure the expression and secretion of 10H10 antibody against tick-borne encephalitis virus in mammalian cells. The vector has the nucleotide sequence of the following SEQ ID NO: 1, size 6523 b.p. and contains elements according to the physical and genetic map shown in FIG. 1. 10H10ch chimeric antibody against tick-borne encephalitis virus has the amino acid sequences of the heavy chain variable fragments of SEQ ID NO: 2 and light chain SEQ ID NO: 3 and is intended for the creation of therapeutic medicinal products against the tick-borne encephalitis virus. The antibody has a wide cross-activity against other flaviviruses. The invention can be used in genetic engineering, biotechnology and medicine.
EFFECT: obtaining 10H10ch chimeric full-length human antibody against tick-borne encephalitis virus produced by a recombinant strain of CHO-K1-10H10ch Chinese hamster ovary cell line and having broad cross-functional activity against tick-borne encephalitis, Zika, dengue, West Nile, yellow fever viruses.
3 cl, 2 dwg, 1 tbl, 3 ex
| Title |
Year |
Author |
Number |
| pVEAL2-9E2ch-SCFv PLASMID GENETIC CONSTRUCT, STRAIN OF RECOMBINANT CELL LINE CHO-K1-9E2ch AND CHIMERIC SINGLE-CHAIN ANTIBODY 9E2ch AGAINST WEST NILE VIRUS PRODUCED BY THE SPECIFIED STRAIN OF CELL LINE CHO-K1-9E2ch WITH HIGH AFFINITY FOR NEONATAL FcRn RECEPTOR |
2022 |
- Nesmeyanova Valentina Sergeevna
- Shanshin Daniil Vasilevich
- Isaeva Anastasiya Aleksandrovna
- Protopopova Elena Viktorovna
|
RU2801532C1 |
| RECOMBINANT PLASMID DNA pCLm4/hygro-14D5 CODING POLYPEPTIDE WITH LIGHT CHAIN PROPERTIES OF CHIMERIC ANTIBODY TO TICK-BORNE ENCEPHALITIS VIRUS, AND RECOMBINANT PLASMID DNA pCHm2-14D5 CODING POLYPEPTIDE WITH HEAVY CHAIN PROPERTIES OF CHIMERIC ANTIBODY TO TICK-BORNE ENCEPHALITIS VIRUS, CHIMERIC ANTIBODY PROVIDING ACUTE PREVENTION OF TICK-BORNE ENCEPHALITIS IN MICE |
2013 |
- Tikunova Nina Viktorovna
- Bajkov Ivan Konstantinovich
- Matveev Andrej Leonidovich
- Babkina Irina Nikolaevna
- Matveev Leonid Ehduardovich
- Rikhter Vladimir Aleksandrovich
|
RU2550252C1 |
| INTEGRATIVE PLASMID VECTOR pVEAL3-Lassa-Trim, PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT SURFACE GLYCOPROTEIN GPC OF Lassa VIRUS IN MAMMALIAN CELLS, RECOMBINANT CELL LINE STRAIN CHO-K1-GPC-LASV AND RECOMBINANT PROTEIN GPC-LASV PRODUCED BY SAID CELL LINE STRAIN CHO-K1-GPC-LASV |
2023 |
- Aripov Vazirbek Salakhiddinovich
- Isaeva Anastasiya Aleksandrovna
- Pyankov Oleg Viktorovich
- Pyankov Stepan Aleksandrovich
- Ilichev Aleksandr Alekseevich
- Shcherbakov Dmitrij Nikolaevich
- Volkova Natalya Vyacheslavovna
|
RU2817423C1 |
| INTEGRATIVE PLASMID VECTOR PVEAL3-GP-MARV-TRIM, PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT SURFACE GLYCOPROTEIN GP OF MARBURG VIRUS IN MAMMALIAN CELLS, RECOMBINANT STRAIN OF CHO-K1-GP-MARV CELL LINE AND RECOMBINANT GP-MARV PROTEIN PRODUCED BY SPECIFIED STRAIN OF CHO-CELL LINE K1-GP-MARV AND USED TO OBTAIN IMMUNOBIOLOGICAL MEDICINAL PRODUCTS |
2023 |
- Mordvinova Ekaterina Denisovna
- Nesmeyanova Valentina Sergeevna
- Nikitin Vladimir Nikolaevich
- Shanshin Daniil Vasilevich
- Volkova Natalya Vyacheslavovna
- Shcherbakov Dmitrij Nikolaevich
|
RU2812356C1 |
| RECOMBINANT PLASMID VECTOR pET32-WNV-DIII, PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT DOMAIN III OF STRUCTURAL GLYCOPROTEIN E OF WEST NILE VIRUS IN ESCHERICHIA COLI CELLS, ESCHERICHIA COLI BL21(DE3)-DIII-WNV CELL LINE STRAIN AND DIII-WNV RECOMBINANT PROTEIN FOR PREPARING IMMUNOBIOLOGICAL PREPARATIONS |
2024 |
- Nesmeyanova Valentina Sergeevna
- Shanshin Daniil Vasilevich
- Isaeva Anastasiya Aleksandrovna
- Volosnikova Ekaterina Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
|
RU2821341C1 |
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2023 |
- Nesmeyanova Valentina Sergeevna
- Merkuleva Yuliya Aleksandrovna
- Isaeva Anastasiya Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
- Volkova Natalya Vyacheslavovna
- Belenkaya Svetlana Valerevna
- Borgoyarkova Mariya Borisovna
- Volosnikova Ekaterina Aleksandrovna
- Esina Tatyana Igorevna
- Zajkovskaya Anna Vladimirovna
- Pyankov Oleg Viktorovich
- Ilichev Aleksandr Alekseevich
|
RU2816175C1 |
| RECOMBINANT PLASMID DNA pFLAG-sc14D5a-Rm7 ENSURING SYNTHESIS OF HYBRID PROTEIN sc14D5a-Rm7, BACTERIA STRAIN Escherichia coli - PRODUCER OF HYBRID PROTEIN sc14D5a-Rm7, AND HYBRID PROTEIN sc14D5a-Rm7, COMBINING PROTEIN E OF TICK-BORNE ENCEPHALITIS MICE AND HAVING BIO-LUMINESCENCE ACTIVITY |
2014 |
- Frank Ljudmila Alekseevna
- Tikunova Nina Viktorovna
- Burakova Ljudmila Petrovna
- Bajkov Ivan Konstantinovich
- Kudrjavtsev Aleksandr Nikolaevich
- Morozova Vera Vital'Evna
|
RU2565545C1 |
| INTEGRATIVE PLASMID VECTOR PVEAL2-S-RBD, PROVIDING THE EXPRESSION AND SECRETION OF THE RECOMBINANT RECEPTOR-BINDING DOMAIN (RBD) OF THE SARS-COV-2 CORONAVIRUS IN MAMMALIAN CELLS, THE RECOMBINANT CHO-K1-RBD CELL LINE STRAIN AND THE RECOMBINANT SARS-COV-2 RBD PROTEIN PRODUCED BY THE SPECIFIED STRAIN OF THE CELL LINE CHO-K1-RBD |
2021 |
- Merkuleva Yuliya Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
- Belenkaya Svetlana Valerevna
- Isaeva Anastasiya Aleksandrovna
- Nesmeyanova Valentina Sergeevna
- Shanshin Daniil Vasilevich
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- Ilichev Aleksandr Alekseevich
- Karpenko Larisa Ivanovna
|
RU2752858C1 |
| TETRAVALENT VECTOR VACCINE AND METHOD OF ITS USE FOR PREVENTION OF DENGUE FEVER |
2023 |
- Yudin Sergej Mikhajlovich
- Bogoslovskaya Elena Vladimirovna
- Glazkova Dina Viktorovna
- Mintaev Ramil Rafailovich
- Orlova Olga Vladimirovna
- Shipulin German Aleksandrovich
- Skvortsova Veronika Igorevna
|
RU2812251C1 |
| METHOD OF PREPARING IMMUNOGENIC COMPOSITION BASED ON THREE FUSION PROTEINS OF SHELL OF TICK-BORNE ENCEPHALITIS VIRUS DEFINING BELONGING TO SIBERIAN (DBD2-D3S), EUROPEAN (DBD2-D3E) AND FAR EAST (DBD2-D3D) VIRUS SUBTYPES; RECOMBINANT PLASMIDS pDBD2-D3S, pDBD2-D3E AND pDBD2-D3D; PRODUCER STRAINS Escherichia coli M15 [pREP4]; CHIMERIC PROTEINS AND THEIR APPLICATION |
2014 |
- Ljashchuk Aleksandr Mikhajlovich
- Aksenova Ekaterina Ivanovna
- Ershova Anna Stepanovna
- Grunina Tat'Jana Mikhajlovna
- Bartov Mikhail Sergeevich
- Savina Dar'Ja Mikhajlovna
- Manukhina Marija Sergeevna
- Gudov Vladimir Petrovich
- Semikhin Aleksandr Sergeevich
- Gra Ol'Ga Alekseevna
- Tkachuk Artem Petrovich
- Galushkina Zoja Mikhajlovna
- Karjagina-Zhulina Anna Stanislavovna
- Lunin Vladimir Glebovich
- Gintsburg Aleksandr Leonidovich
|
RU2560588C1 |
