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IPC

C12N5/16(2006-01-01)

C12N15/24(2006-01-01)

C12N15/85(2006-01-01)

(21) (22)

Application

2012145810/10, 2012-10-29

(24)

Start date

2012-10-29

(22)

Actual filing date

2012-10-29

(45)

Published

2015-09-10

(72)

Inventor

Pigareva Natal'Ja Vasil'EvnaIshchenko Aleksandr MitrofanovichPetrov Aleksandr VladimirovichKlimov Nikolaj Anatol'EvichSimbirtsev Andrej Semenovich

(73)

Holder

Federal'Noe Gosudarstvennoe Unitarnoe Predprijatie Nauchno-Issledovatel'Skij Institut Osobo Chistykh Biopreparatov" Federal'Nogo Mediko-Biologicheskogo AgentstvaRossijskaja Federatsija V Litse Ministerstva Obrazovanija I Nauki Rossijskoj Federatsii

CULTURE CELL STRAIN CHO-IL7/13 PRODUCING HUMAN INTERLEUKIN-7 Russian patent published in 2015 - IPC C12N5/16 C12N15/24 C12N15/85

Abstract RU 2562169 C2

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and concerns the cell strain CHO-IL7/13. The characterised strain is produced by transfection of the cells CHO^dhfr by pIPvES-DHFR/IL7 plasmid containing human interleukin-7 gene. pIPvES-DHFR/IL7 plasmid has been synthesised by primer amplification of human interleukin-7 complementary DNA: ecoIL CCTGAATTCCACCATGTTCCATGTTTCTTTTAG containing EcoRI restriction site and Kozak sequence, and bamIL CCAGGATCCTCAGTGTTCTTTAGTGCCCATC containing BamHI restriction site that is followed by cloning according to the restriction site data into pIRES-DHFR vector.

EFFECT: presented strain has a higher producing ability of human recombinant interleukin-7 and can be used for treating the number of pathological conditions associated with the low content of B- and T-lymphocytes.

4 dwg, 3 ex

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RU 2 562 169 C2

Authors

Pigareva Natal'Ja Vasil'Evna

Ishchenko Aleksandr Mitrofanovich

Petrov Aleksandr Vladimirovich

Klimov Nikolaj Anatol'Evich

Simbirtsev Andrej Semenovich

Dates

2015-09-10—Published

2012-10-29—Filed