METHOD OF CRYOCONSERVATION OF MULTIPOTENT MESENCHYMAL STROMAL CELLS Russian patent published in 2011 - IPC C12N5/00 A01N1/02 

FIELD: medicine.

SUBSTANCE: method of cryoconservation of multipotent mesenchymal stromal cells (MMSC) includes preparation of culture mixture in culture medium, mixing cell culture in culture medium with cryoprotector, step-by-step controlled cooling and freezing mixture of culture medium with cryoprotector to storage temperature and further storage of frozen mixture at low temperatures. As cryoprotector for mixture saturation used is xenon gas, introduced till saturation is achieved into prepared mixtute of cell culture MMSC in DMEM medium, which is in open cryo test tubes by blowing culture medium with xenon at 0°C with further cooling and formation of primary monolith of culture medium with cryoprotector, directed later at cooling to storage temperature.

EFFECT: invention ensures preservation of cells in cryopreservation.

4 cl, 2 dwg, 2 tbl