FIELD: medicine.
SUBSTANCE: what is presented is a kit of reagents for immune-enzyme assay of the endogenous bioregulator antibodies in blood serum for detecting addiction disorders by two-site enzyme immunoassay in human blood serum in vitro. The kit comprises a 96-well demountable stripped plate with its surface sequentially coated by all conjugates: βendorphins, serotonin, dopamine, histamine, orphanin and angiotensin, as well as containers containing positive and negative reference samples, horseradish peroxidase-labeled anti-species anti-human antibodies, phosphate-buffered saline containing Tween 20, a substrate buffer solution, a tetramethyl benzidine substrate solution, and a stop solution.
EFFECT: more accurate detection of the addiction diseases, including alcoholism, drug addiction, chemical abuse, food addiction and gambling by using the kit of six labels.
3 tbl, 9 ex
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Authors
Dates
2013-10-10—Published
2011-11-15—Filed