FIELD: biotechnology, method for cryoconservation of biological cells for prolonged storage.
SUBSTANCE: claimed method includes selection of fertilized dog's tail Beroe ovata eggs in step of 2-4 blastomeres. Then eggs are placed in barrier solution, frozen by dipping in liquid nitrogen followed by storage therein. Further eggs are defrosted followed by washing thereof in washing solution. Sea water used as physiological medium for barrier and washing solutions is preliminary sterilized by filtration through membrane filter.
EFFECT: increased yield of non-damaged dog's tail eggs being capable of further development.
2 tbl, 8 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING CRYOPROTECTING AGENT FOR CRYOMEDIA | 2002 |
|
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| METHOD FOR PRESERVING VIABILITY OF HEMATOPOIETIC STEM CELLS OF UMBILICAL BLOOD AFTER WASHING FROM CRYOPROTECTANT | 2023 |
|
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| METHOD FOR CATTLE OOCYTE FREEZING | 2018 |
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| METHOD FOR PRIMARY SELECTION OF MATERIALS THAT HAVE PROJECTIVE FEATURES | 1992 |
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| UNGULATE EMBRYO CRYOPRESERVATION | 2016 |
|
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| TRANSGENESIS DUE TO INTRACYTOPLASMATIC SPERMATIC INJECTION IN MAMMALIANS | 1999 |
|
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| METHOD FOR CRYOCONSERVATION OF CELLS IN SEA INVERTEBRATES | 2006 |
|
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| METHOD FOR CULTIVATION OF OITHONA DAVISAE MARINE CYCLOPOID COPEPODS | 2022 |
|
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| METHOD FOR REDUCING TOXICITY OF CRYOPRESERVATION SOLUTION BASED ON DIMETHYL SULFOXIDE AFTER THAWING OF HEMATOPOIETIC STEM CELLS | 2020 |
|
RU2744614C1 |
| METHOD FOR GROWING NORMAL DESCENDANTS FROM OOCYTES INJECTED WITH SPERMATOZOA TREATED BY DRYING WITH FREEZING | 1999 |
|
RU2244525C2 |
