METHOD FOR SEPARATING X-CHROMOSOME-CARRYING SPERMATIC CELLS AND Y-CHROMOSOME-CARRYING SPERMATIC CELLS Russian patent published in 2007 - IPC A61D19/02 

FIELD: medicine, artificial insemination.

SUBSTANCE: the innovation consists of the following stages: selection of spermatic cells from a male of a certain mammalian type except a human being; introduction of spermatic cells into the flow of fluid medium; analysis of spermatic cells taken by the flow of fluid medium; detection of DNA quantity in the nuclei of spermatic cells; irradiation of dyed DNA in the nuclei of spermatic cells; detecting the fluorescent light transmitted by irradiated dyed DNA being in the nuclei of spermatic cells; differentiation of spermatic cells based upon the quantity of DNA being inside the nuclei of spermatic cells located in the present drop; electrostatic deviation of every drop; separate accumulation of drops based upon the quantity of DNA inside the nuclei of spermatic cells captured into each drop; separation of differentiated spermatic cells into X-chromosome-carrying populations and Y-chromosome-carrying ones; and obtaining the populations of intact X-chromosome-carrying and Y-chromosome-carrying spermatic cells at purity degree being above 90% for each. The innovation enables to improve separation of spermatozoa and increase its velocity.

EFFECT: higher efficiency.

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